24th Meeting of the EPPO Panel on Diagnostics in Bacteriology

Teleconference 2022-01-25/27

 

 

The EPPO Panel on Diagnostics in Bacteriology met by videoconference. Only selected points of the agenda prepared for the original meeting (before it needed to be changed to a teleconference) were considered.

 

 

 

 

 

Revised EPPO Diagnostic Protocols reviewed during the meeting

The Panel reviewed 5 revised Diagnostic Protocols.

 

PM7/023 Xanthomonas axonopodis pv. dieffenbachiae

A revised version of the Diagnostic Protocol was prepared by the EPPO Secretariat with the support of two experts from ILVO (BE) and CIRAD (FR). The Panel reviewed the main text and the appendices of the draft revision of the Diagnostic Protocol. The EPPO Secretariat will finalize the document based on the Panel discussion and the feedback of some experts. The new version will be sent for country consultation, if possible, in the first semester of 2022.

 

PM 7/024 Xylella fastidiosa

A revised version of the Diagnostic Protocol prepared by a drafting team composed of experts from INRAE (FR), ANSES (FR), NIB (SI), UNIBA (IT), CREA (IT), IVIA (ES), CNR (IT), NVWA (NL), JKI (DE), and AGES (AT) was presented to the Panel. A new version that takes into account the discussion of the Panel will be prepared by the EPPO Secretariat and the Drafting Team, sent to the Panel and then sent for country consultation.

 

PM 7/044 Xanthomonas citri pv. citri and Xanthomonas citri pv. aurantifolii

A revised version of the Diagnostic Protocol prepared by a drafting team composed of experts from CIRAD (FR), ANSES (FR), IRD (FR), INIA (ES), UNICT (IT) was presented to the Panel. Two experts from NVWA and AGES shared their experience with additional tests (Immunofluorescence and the conventional PCR from Fonseca et al. (2019)) not included in the Diagnostic Protocol. The Panel extensively discussed the advantages and disadvantages of including these tests for the identification of Xanthomonas citri pv. aurantifolii in comparison with the real-time PCR from Mavrodieva et al. (2004). The drafting team will be reconvened to finalize the revision of the Diagnostic Protocol. The new version will be sent for country consultation, if possible, in the first trimester of 2022.

 

PM 7/065 Xanthomonas fragariae

A revised version of the Diagnostic Protocol prepared by a drafting team composed of experts from Fera (GB) and ZHAW (CH) was presented to the Panel.The revision was initially prepared to align the EPPO Diagnostic Protocol with the IPPC Diagnostic Protocol adopted in 2016. However, the draft protocol also includes other tests identified in the framework of the EU COST Action ‘Integrating science on Xanthomonadaceae for integrated plant disease management in Europe (EuroXanth)’. A new draft of the Diagnostic Protocol that takes into account the discussion of the Panel will be prepared by the EPPO Secretariat and the Drafting Team, sent to the Panel and then sent for country consultation.

 

PM7/110 Xanthomonas spp. causing bacterial spot of tomato and sweet pepper

A revised version of the Diagnostic Protocol was prepared by the EPPO Secretariat. The Panel extensively discussed the possibility of including MLSA tests for the identification of this complex of species and concluded that further work was needed before such tests are included in the protocol. The Panel reviewed the main text and the appendices of the draft revision of the Diagnostic Protocol. The EPPO Secretariat will revise the document based on the Panel discussion and the feedback of some experts and with the help of some Panel members. The new version will be sent for country consultation, if possible, in the first semester of 2022.

 

 

Other revisions

PM 7/043 Pseudomonas syringae pv. persicae

A revised version of the Diagnostic Protocol was prepared by the EPPO Secretariat with the support of an expert from FERA (GB). The Panel did not review the protocol during the Panel meeting due to lack of time. It will be reviewed in the first trimester of 2022 and a new version will be sent for country consultation.

 

 

New EPPO Diagnostic Protocols reviewed during the meeting

The Panel reviewed one new Diagnostic Protocol.

 

Dickeya spp. and Pectobacterium spp.

The Diagnostic Protocol prepared by a drafting team composed of experts from FN3PT (FR), IVIA (ES), FERA (GB), SASA (GB), AGES (AT), UNIMORE (IT), NAK (NL) and ILVO (BE) was presented to the Panel. A new draft of the Diagnostic Protocol that takes into account the discussion of the Panel will be prepared by the Drafting Team and sent for country consultation.

 

 

Plans for future new diagnostic protocols

Proposals were made to develop a Standard on the use of the matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometry method for the identification of plant pathogenic bacteria and a Diagnostic Protocol on Curtobacterium flaccumfaciens pv. poinsettiae.

 

 

Next Panel meeting

The next meeting of the Panel on Diagnostics in Bacteriology will be held in Lithuania in Autumn 2022.

 

Participants

The composition of the EPPO Panel on Diagnostics in Bacteriology can be found on the EPPO website.