EPPO activities on diagnostics
Since 1998, EPPO has established a work programme in the area of diagnostics to harmonize procedures across the EPPO region. This programme mainly focuses on the diagnostics of pests which are regulated as quarantine pests. The different activities conducted in this framework are described below.
Panel on Diagnostics and Quality Assurance
Panel on Diagnostics in Nematology
(Wageningen, NL, 2017-09-06/08)
Panel on Diagnostics in Entomology
EPPO diagnostic Standards
More than 130 EPPO diagnostic Standards have been published
Since 1998, more than 130 EPPO diagnostic Standards have been prepared by EPPO. All have been published in the EPPO Bulletin and are also stored in the EPPO Global Database. This set of Standards includes horizontal and pest-specific Standards.
- Horizontal Standards include Standards related to quality assurance issues, Standards on how to perform specific methods, such as ELISA test for viruses, DNA barcoding as an identification tool for a number of regulated pests etc.
- Pest-specific diagnostic Standards (Diagnostic protocols) are related to specific pests (a single species or groups of species) and provide the guidance necessary for an expert or competent staff who are specifically trained to detect and positively identify these pests by a single test or combination of tests.
Two revised versions of Diagnostic Standards available:
- PM 7/40 (5) Globodera rostochiensis and Globodera pallida
- PM 7/21 (3) Ralstonia solanacearum, R. pseudosolanacearum and R. syzygii (Ralstonia solanacearum species complex)
These two revised versions are made available here prior to their publication in the EPPO Bulletin, as revision of regulations on potato pests is in progress in a major part of the region including elements on diagnostics.
How EPPO diagnostic Standards are prepared?
The preparation of Standards involves close collaboration between different EPPO Panels involved in diagnostics. These Panels are composed of diagnostic experts nominated by the NPPOs of the EPPO member countries. The EPPO Panels involved in diagnostics are the following (click on the links to view their composition):
- Panel on Diagnostics and Quality Assurance
- Panel on Diagnostics in Bacteriology
- Panel on Diagnostics in Entomology
- Panel on Diagnostics in Nematology
- Panel on Diagnostics in Virology and Phytoplasmology
- Panel on Diagnostics in Mycology
Drafts of pest specific Diagnostic Standards (Diagnostic protocols) are initially prepared by an individual expert or a group of experts (drafting team) according to a common format which ensures that the draft contains all necessary information to detect and positively identify a particular pest (instructions for authors ). Whenever available, validation data is also provided for the different tests included in the diagnostic protocols. The draft protocols are reviewed by the relevant Panels and submitted to a consultation phase among all EPPO member countries to ensure their wide acceptance.
For horizontal Standards no specific format exist but as pest Specific ones, draft are usually prepared by an individual expert or a drafting group.
As it is the case for all EPPO Standards, diagnostic Standards are officially approved and then published in the EPPO Bulletin.
Evaluation of the need for revision
For each pest-specific diagnostic protocol one (or more) expert from the relevant Panel is responsible for reviewing the new literature for this pest before each Panel meeting and informing the EPPO Secretariat if there is a need to revise the protocol (e.g. due to new improved tests being developed which should be included or changes in taxonomy).
EPPO pictorial glossary of morphological terms in nematology
Since 2013, the EPPO Panel on Diagnostic in Nematology has been developing a pictorial glossary of morphological terms in nematology. This document lists all nematological terms used in the EPPO diagnostic protocols on plant parasitic nematodes with their definitions.
Pictorial Glossary of Morphological Terms in Nematology (2020 version)
EPPO databases on diagnostics
EPPO database on diagnostic expertise
In 2004, EPPO Council stressed that the implementation of phytosanitary regulations for quarantine pests was jeopardized by decreasing knowledge in plant protection and made an official declaration (Plant Health Endangered, also known as the ‘Madeira declaration’). The Panel on Diagnostics proposed that an inventory should be made of the available expertise on diagnostics in Europe. A database on Diagnostic Expertise was then created to allow identification of experts who could provide diagnosis of regulated species or help in the identification of new or difficult species. Experts from more than 100 diagnostic laboratories of the EPPO region have provided details about the pests they can diagnose and the methods they use. EPPO member countries are encouraged to continue to provide data on their laboratories and expertise.
In May 2020, EPPO launched a renewed version of the database. The new version was prepared in the framework of an EU funded project VALITEST and the outcome of the redesign is a more searchable and user-friendly database.
EPPO-Q-bank is a database to support diagnostic activities on plant pests which was transferred to EPPO and officially launched in May 2019. The primary aim of EPPO-Q-bank is to host sequence data for a number of pests, as well as to provide information on biological material, in particular from where this material can be obtained. Information is provided per discipline (arthropods, bacteria, fungi, nematodes, phytoplasmas, viruses & viroids, and invasive plants).
Workshops and Conferences on Diagnostics
Since 1985, EPPO has organized a series of workshops and conferences on diagnostics.
- Conferences on diagnostics (1985, 1994, 2000 and 2012 in NL - 2009 in GB - 2015 in FR)
- Workshops on Quality Assurance (2007 in DK - 2009 and 2014 in GB)
- Workshop for Heads of laboratories (2011 in AT - 2013 in TN - 2015 in IT)
- Workshop on 'cut-off data' for real-time PCR (2013 in FR)
- Q-collect 2nd project Workshop (Rome, 2015-09-08/09)
3rd Workshop for Heads of Laboratories (Rome, 2015-09-08/11)
- Testa - EPPO Conference on diagnostics for plant pests and associated workshops (Angers, FR, 2015-11-30/12-04)
Workshop on Flexible Scope (Wageningen, NL, 2017-06-26/28)
EPPO Workshop on Maintenance of Nematode Collections (Wageningen, NL, 2017-09-05/06)
Workshop on the use of Next Generation Sequencing technologies for plant pest diagnostics (Bari, IT, 2017-11-22/23)
Partnerships and special projects
EPPO and IPPC Diagnostic protocols
EPPO Panels are reviewing Diagnostic protocols prepared at IPPC level to prepare regional positions to be used by EPPO Countries during the IPPC consultations.
Ms Petter (EPO Assistant Director) is also invited to attend the IPPC Technical Panel on Diagnostic Protocols (and several meetings of that Panel have been hosted in the EPPO Headquarters).
IPPC Technical Panel on Diagnostic Protocols (TPDP) members and participants
(EPPO headquarters, Paris, February 2018)
Since 2014, EPPO is hosting Euphresco, a network of organizations (e.g. NPPOs, research institutes) funding and coordinating research in plant health. Many projects funded by the Euphresco network relate to diagnostic issues.
EPPO has a close collaboration with the European Commission (EC) and an expert from the EC attends all EPPO Diagnostic Panels.
European Co-operation for Accreditation (EA)
The European Co-operation for Accreditation (EA) is the European network of nationally recognised accreditation bodies located in the European geographical area. In 2009, a joint communiqué was signed by EPPO and EA stating that 'EA will recommend that assessors from Accreditation Bodies take note of EPPO documents when evaluating plant pest diagnostic laboratories'. Since 2011-01 EPPO is a 'recognized stakeholder' for EA. A revised version of this communiqué was signed in October 2018.
Revised joint communiqué between EPPO and EA (2018-10-10)
European Association of Phytobacteriologists
EPPO collaborates with the European Association of Phytobacteriologists (EAP) and the Panel on Diagnostics in Bacteriology and the EAP regularly have back to back meetings.
European Mycological Network
EPPO collaborates with the European Mycological Network (EMN) and the Panel on Diagnostics in Mycology and the EMN regularly have back to back meetings.
In addition to its regular programme of activities, EPPO participates in various projects that are conducted over a fixed period of time and that may be funded by the Organization itself, part of its members, or other organizations. In the field of diagnostics, EPPO has participated in several projects, such as Q-detect, Q-collect and TESTA. EPPO is currently a partner in the EMPHASIS, XF-ACTORS and VALITEST projects.
- EPPO (2020) Instructions to authors on the format and content of a diagnotic protocol.
- EPPO Global Database
- EPPO Reporting Service
- IPPC – ISPMs on diagnostics (DPs)
- National Plant Biosecurity Diagnostic Network Australian diagnostic resources for priority pests
- EPDIA - Diagnostic Kits Database
- Petter F, Suffert M (2010) Survey on the use of tests mentioned in EPPO diagnostic protocols. Bulletin OEPP/EPPO Bulletin 40, 121–126.
- Roy AS, Petter F, Griessinger D (2010) EPPO database on diagnostic expertise: http://dc.eppo.org. Bulletin OEPP/EPPO Bulletin 40, 127–130.