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Panel Diagnostics in Nematology

10th Meeting of the Panel on Diagnostics in Nematology

Vienna, 2016-09-07/09


The Panel met in Vienna on 2016-09-07/09 at the kind invitation of AGES (Austrian Agency for Health and Food Safety) (download a list of participants ). The Panel reviewed several protocols for which revision is in progress.


Globodera pallida and G. rostochiensis
A fourth revision of the Standard is in preparation. The draft revision was sent for country consultation in 2015 and comments received needed a face to face discussion before the document could be finalized. In particular one country suggested the addition of a new key to potato cyst nematode species. This suggestion was welcomed and a small group of experts will prepare this key. The Standard is expected to be sent for the final stages of approval by the end of 2016.

Aphelenchoides besseyi
An IPPC protocol on Aphelenchoides species was adopted in August 2016 and the Panel agreed that the EPPO protocol should be aligned to this protocol (in particular keys to the genus Aphelenchoides and how to distinguish A. besseyi from the other species in the genus). It was however considered that drawings of species currently included in the EPPO protocol which are not in the IPPC protocol are valuable and should be maintained as supplementary information. The revision should be sent for country consultation by the end of 2016.

Ditylenchus dipsaci and D. destructor
An IPPC diagnostic protocol on Ditylenchus dipsaci and D. destructor was approved in January 2016. A revision of the EPPO protocol to align it with the IPPC protocol is in preparation which should be sent for country consultation by the end of 2016.

Xiphinema americanum sensu lato
An IPPC diagnostic protocol on Xiphinema americanum sensu lato was approved in January 2016. A revision of the EPPO protocol to align it with the IPPC protocol has been prepared. Since the adoption of the IPPC protocol, new species have been described and have been added to the keys in the EPPO protocol. The revised version should be sent for country consultation by the end of 2016.

Heterodera glycines
A revision of the protocol was reviewed and the Panel recommended that contact should be made with scientists in North America to gather more information on available molecular tests. The revised protocol should be sent for country consultation by the end of 2016.

Bursaphelenchus xylophilus
An IPPC diagnostic protocol on Bursaphelenchus xylophilus was approved in January 2016. The Panel noted that the current version of the EPPO protocol is in line with the IPPC protocol although a reference to the LAMP test (Leal et al 2015) could be made as well as to the new EPPO horizontal Standard on barcoding. It was also suggested that pictures of Monochamus infested wood could be added to the EPPO Global Database and referred to. An addendum to the EPPO Standard will be prepared by the EPPO Secretariat.

Other topics
The Panel reviewed the Pictorial Glossary and suggested some changes. A revised version will be posted on the website by the end of September 2016.
The Panel exchanged information on nematodes detected for the first time in the region (Meloidogyne graminicola in Italy under eradication and B. luxuriosae a non-pathogenic nematode detected in Portugal).
The Panel noted the recent publication suggesting a synonymization of Meloidogyne ethiopica with M. luci (Jansen et al., 2016).
The Panel also raised a number of horizontal issues which will be discussed further at the Panel on Diagnostics and Quality Assurance in January 2017, for example the requirement for certain controls for PCR.
The Panel discussed the outcomes of the Q-collect project and considered that it is essential that information is shared between the laboratories on how to maintain collections in nematology. It was agreed that a Workshop on this topic should be organised and could be held back-to-back with the next Panel meeting.

Alignment of EPPO protocols to IPPC protocols
When an IPPC protocol is approved an alignment of the corresponding EPPO protocol is undertaken. The alignment consists in browsing the content of the IPPC protocol and identifying changes to be made in EPPO protocols. This means that the EPPO format is kept but that the content (except in very exceptional circumstances) is consistent.
When the first IPPC protocols were developed the EPPO Working Party on Phytosanitary Regulations considered that the EPPO protocols should not be withdrawn but rather aligned in particular to focus on regional experience (tests for which experience exists in the region are described with more detail than those for which no expertise exists). Morphological descriptions are directly aligned with the IPPC protocol (exceptions will be made when the range of species covered is not the same etc.). However for molecular tests it is considered that the EPPO format should be used as more details are required in the EPPO format in particular under the performance criteria.

Next meeting
The next Panel will be organised in the Netherlands at the kind invitation of the National Reference Center (NRC), Wageningen, NL. The Panel will be held back-to-back with a Workshop on how to maintain collections in nematology. The provisional dates are for the week of the 4th of September 2017 (to be confirmed).



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