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16th Meeting of the Panel on Diagnostics in Bacteriology

Sofia, 2011-10-24/26


The Panel on Diagnostics in Bacteriology met in Sofia at the kind invitation of the Central Laboratory for Plant Quarantine, part of the Bulgarian Food Safety Agency. This was the third joint meeting with the European Association of Phytobacteriologists (EAP).

The Panel continued its work on the preparation of diagnostic protocols. To date, 16 Diagnostic Protocols for bacterial diseases, and 2 General standards on Bacteriology techniques have been published.

The following protocols (in preparation) were reviewed by the Panel:

  • Pseudomonas syringae pv. actinidiae
  • Xanthomonas vesicatoria (see new scope below)
  • Citrus greening (Candidatus Liberibacter)
  • Xanthomonas axonopodis pv. allii

Participants - View more pictures >

The Panel agreed that the protocol on Pseudomonas syringae pv. actinidiae should be revised after the results of the test performance study are available. The Panel agreed with the suggested modification of the scope of the protocol on Xanthomonas vesicatoria to ‘Xanthomonas spp. causing bacterial spot of tomato and sweet pepper’. The Panel agreed that more details of performance criteria should be added to the protocol on Citrus greening, that information should be added on the testing of vectors and that the protocol needs to be aligned to the format in the instructions for authors. The Panel agreed that revised versions of these 3 protocols should be prepared for the end of January, reviewed by the Panel via e-mail consultation, and then sent for country consultation in early 2012 if possible.
The Panel agreed that the EPPO Secretariat should discuss with the author of the protocol on Xanthomonas axonopodis pv. allii whether they insist on patenting the primers. It was agreed that the Panel should review this protocol and send comments to the EPPO Secretariat to discuss these with the author.
The following protocols (revisions) were reviewed by the Panel:

  • Clavibacter michiganensis subsp. michiganensis
  • Erwinia amylovora

The Panel discussed the proposed revision of the protocol on Clavibacter michiganensis subsp. michiganensis, and the numerous comments made on it by the members of the Panel, in particular the Panel discussed: the objectives of the revision (to revise seed testing methods); the 2 procedures proposed (it was agreed to state that these have been independently validated but that equivalence has not been shown); the media used (both the new semi-selective media and the non-selective media to be included); the tests used; and the need for spiking. Specific members of the Panel agreed to revise different sections of the protocol, following the decisions made at the meeting, and it was agreed that if these sections could be received and compiled in January then this protocol could be sent for country consultation in early 2012.
The Panel agreed that a revised version of the protocol on Erwinia amylovora should be prepared, and that performance criteria should be added, and that this should be sent for country consultation in 2012.

The improvements to the EPPO database on diagnostic expertise were presented (links to accreditation certificates; inclusion of technical auditors and technical experts).
The latest version of the EPPO database Plant Quarantine data Retrieval system (PQR) was presented to the Panel along with the work towards the EPPO Global database. This work was very warmly received.
A presentation of the work done in bacteriology as part of the QBOL project was made by Ms Bergsma-Vlami.

Next meeting
The next meeting of the Panel on Bacterial Diseases is scheduled for 2012-10-20/23 in Angers (FR), at the kind invitation of ANSES, Plant Health Laboratory.


Participant List

The State Plant Service under the Ministry of Agriculture, Phytosanitary Research Laboratory, Sukileliu str. 9a, LT-11351 Vilnius, Lithuania - Tel +370-52611802 - Fax +370-52616801 -

The Danish Plant Directorate (Plantedirektoratet), Laboratory for Diagnostics on Seed, Plants and Fodder, Skovbrynet 20, DK-2800 Lyngby, Denmark - Tel +45-45263845 - Fax +45-45263600 -

DRENOVA Natalia (Ms)
Bacteriology Laboratory, Federal State Entreprise "All-Russian Plant Quarantine Centre", Pogranichnaya str. 32, 140150 Moscow Oblast, Ramensky Region, Bykovo-2, Russia - Tel +7-916-108-80-58 - Fax +7-4952237241 - drenova@mail.r

DREO Tanja (Ms)
National Institute of Biology, Vecna pot 111, 1000 Ljubljana, Slovenia - Tel +386-59232806 - Fax +386-12573847 -

DUFFY Brion (Mr)
Agroscope Changins-Wädenswil ACW, Schloss 1, CH-8820 Wadenswil, Switzerland - Tel +41-447836416 - Fax +41-447836305 -

Food and Environment Research Agency, Sand Hutton, YO41 1LZ York, United Kingdom - Tel +44 1094 462334 -

Austrian Agency for Health and Food Safety (AGES), Institute for Plant Health, Spargelfeldstrasse 191, A-1226 Vienna, Austria - Tel +43-5055533343 - Fax +43-5055533303 -

HORKY Jaroslav (Mr)
State Phitosanitary Administration, Division of Diagnostics, Slechtitelu 23, 77900 Olomouc, Czech Republic - Tel +420-585570153 - Fax +420-585227790 -

KOUTZAROVA Eleonora (Ms)
Central laboratory for plant quarantine, 120, N. Moushanov Blvd., 1330 Sofia, Bulgaria - Tel +359-28223381 - Fax +359-28223381 -

LEVY Edna (Ms)
Plant Protection and Inspection Services, P.O Box 78, 50250 Bet-Dagan, Israel - Tel +972-39681534 - Fax +972-39681582 -

LOPEZ Maria (Ms)
IVIA, Apartado Oficial, 46113 Moncada Valencia, Spain - Tel +34-963424000 - Fax +34-963424001 -

MALDUTE Sanita (Ms)
State Plant Protection Service, National Phytosanitary Laboratory,  Lielvardes Str. 36/38, LV-1006 Riga, Latvia - Tel +371-26326274 - Fax +371-67543201 -

MALIK Richard (Mr)
Central Control and Testing Institute of Agriculture, Section of Diagnostics, Osada 281, 04457 Haniska, Slovakia - Tel +421-556930291 - Fax +421-556930291 -

MUELLER Petra (Ms)
Julius Kühn Institut (JKI), Institute for National and International Plant Health, Stahnsdorfer Damm 81, 14532 Kleinmachnow, Germany - Tel +49-3320348377 - Fax +49-3320348385 -

NEMETH Jozsef (Mr)
Central Agricultural Office, Directorate of Plant Protection, Soil Conservation and Agro-environment, Kodo d. 1., 7634 Pecs, Hungary - Tel +36-72552246 - Fax +36-72255940 -

POLIAKOFF Françoise (Ms)
Laboratoire de la santé des végétaux, 7 rue Jean Dixméras, 49044 Angers Cedex 01, France - Tel +33-241199685(nouv standard +33-24120742) - Fax +33-241207430 -

SLETTEN Arild (Mr)
Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, Hogskoleveien 7, N-1432 Aas, Norway - Tel +47-90526644 - Fax +47-64946110 -

STEFANI Emilio (Mr)
Dept. Agricultural and Food Sciences, Via Amendola 2, 42122 Reggio Emilia, Italy - Tel +39-0522522062Mobil phone : +39-3398759502 - Fax +39-0522522053 -

NRL (National Reference Laboratory), Plant Protection Service, P.O. Box 9102, 6700 HC Wageningen, Netherlands - Tel +31651246794 -

Institute of Agricultural and Fisheries Research (ILVO), Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium - Tel +32-92722476 - Fax +32-92722429 -

EAP Observers
Ministry of Economic Affairs, Agriculture and Innovation, Geertjesweg 15, PO Box 9102, 6700 HC Wageningen, Netherlands - Tel +31-317496830 - Fax +31-317421701 -

JANSE Jacob Dirk (Mr)
Dutch General Inspection Service (NAK) Department Laboratory Methods and Diagnosis, PO Box 1115, 6300 BC Emmeloord, Netherlands - Tel +31-527635400 - Fax +31-527635411 -

Central laboratory for plant quarantine, 120, N. Moushanov Blvd., 1330 Sofia, Bulgaria - Tel +359-8024705 - Fax +359-28223381 -

DIMOVA Milena (Ms)
Central laboratory for plant quarantine, 120, N. Moushanov Blvd., 1330 Sofia, Bulgaria - Tel +359-8024716 - Fax +359-28223381 -

PETTER Françoise (Ms)
MCMULLEN Madeleine (Ms)
21 boulevard Richard Lenoir, 75011 Paris, France - Tel +33-145207794 - Fax +33-170766547 -


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