14th Meeting of the EPPO Panel on Bacterial diseases
Izmir (TR), 2009-02-02/04
The Panel on bacterial diseases met at the Bornova Plant Protection Institute in Izmir at the kind invitation of the Turkish NPPO. The meeting was held in connection with the First meeting of the Association of Phytobacteriologists (EAP). The EPPO Secretariat expressed its satisfaction that the two meetings can be held back to back for a mutual benefit.
The Panel continued its work on the preparation of diagnostic protocols. To date, 14 diagnostic protocols for bacterial diseases have been published.
Participants >>
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The following protocols were reviewed:
- A revision of the protocol for Xanthomonas axonopodis pv. diffenbachiae (to include a PCR test).
- A revised draft for Xylophilus ampelinus (to include a real-time PCR test)
Both were considered ready to be sent for country consultation after the meeting with the aim to present them for final approval in 2009 if the EPPO Secretariat is able to address comments made during country consultation together with the authors.
Revised drafts for Clavibacter michiganensis subsp. insidiosus, and Curtobacterium flaccumfaciens pv. flaccumfaciens were also reviewed and considered ready to be sent for country consultation later in 2009 (further work is needed on these protocols).
Regarding Xanthomonas vesicatoria it was noted that recent developments in taxonomy have consequences on the current draft which may need to be adapted. The Panel reviewed the different sections to identify the parts that needed to be adapted.
The Panel agreed to standardise the texts for the Immunofluorescence test (IF), ELISA and pathogenicity tests and experts volunteered to prepare it. A text for IF was prepared immediately after the meeting and after an email consultation with the Panel it was concluded that it should be presented for adoption as a standard in the series PM 7.
Following comments questioning whether pathogenicity tests should always be performed for the identification of a bacterium, the Panel agreed that the inclusion of pathogenicity tests in diagnostic protocols should be considered on a case by case basis.
The Panel was informed of the latest developments concerning the EPPO programme on quality assurance and accreditation and in particular the new standard Specific requirements for laboratories preparing for accreditation for plant pest diagnostic activities. This standard gives guidance on how validation of a test should be performed. It also describes how laboratories can demonstrate that they perform a validated test correctly (verification). The Panel on bacterial diseases suggested that more guidance should be given in this standard on validation of reagents and this suggestion was presented to the EPPO Panel in charge of the development of the standard.
The EAP recommended that Acidovorax avenae pv. citrulli should be added to the EPPO Alert List and should be considered as a priority pest for PRA: this pest has been detected every year to varying degrees of significance in melon production areas nationwide.
The Secretariat informed the EAP that EPPO had initiated a revision of the current Lists of pests recommended for regulation in order to evaluate the pests that should be deleted from these lists. The EAP welcomed the possibility to be associated with this review.
The next meeting of the Panel on bacterial diseases is scheduled for 2010-03-22/26 in Ljubljana, at the kind invitation of Ms Dreo and Ms Ravnikar.
Participants
Baranauskaite Laima (Ms)
State Plant Protection Service, Phytosanitary Research Laboratory, Sukileliu str. 9a, 11351 Vilnius (LT) - +370-52611802 - +370-52616801 - vaatla@vaat.lt
Caffier David (Mr)
Laboratoire National de la Protection des Végétaux, Unité de Bactériologie, 7 rue Jean Dixmeras, 49044 Angers (FR) - +33-241723241 - +33-626752102 - +33-241482285 - david.caffier@agriculture.gouv.fr
Drenova Natalia (Ms)
Bacteriology Laboratory, Federal State Entreprise "All-Russian Plant Quarantine Centre", Pogranichnaya str. 32, 140150 Moscow Oblast, Ramensky region, Bykovo-2 (RU) - +7-495 785 7613 - +7-495 223 7241 – drenova@mail.ru ; vniikr@mail.ru ; filial53@mail.ru
Dreo Tanja (Ms)
National Institute of Biology, Vecna pot 111, 1000 Ljubljana (SI) - +386/12573847 - +386/12573847 - tanja.dreo@nib.si
Duffy Brion (Mr)
Agroscope Changins-Wädenswil ACW, Schloss, Postfach, CH-8820 Wädenswil (CH) - +41-447836416 - +41-447836305 - Brion.Duffy@acw.admin.ch
Gottsberger Richard (Mr)
Austrian Agency for Health and Food Safety (AGES), Spargelfeldstrasse 191, 1226 Vienna (AT)
+43-5055533343 - +43-5055533303 - pflanzengesundheit@ages.at
Horky Jaroslav (Mr)
State Phitosanitary Administration, Division of Diagnostics, Slechtitelu 23, 77900 Olomouc (CZ) - +420-585570153 - +420-585227790 - jaroslav.horky@srs.cz
Lopez Maria (Ms)
IVIA, Carretera Moncada a Naquera km 4.5 Apartado Oficial, 46113 Moncada Valencia (ES) - +34-963424000 - +34-963424001 - mlopez@ivia.es
Malik Richard (Mr)
Central Control and Testing Institute of Agriculture, Section of Diagnostics, Osada 281, 04457 Haniska (SK) - +421-556930291 - +421-556930291 - richard.malik@zoznam.sk; rchrdmalik@yahoo.com
Muller Petra (Ms)
Julius Kühn Institut (JKI), Institute for National and International Plant Health, Stahnsdorfer Damn 81, 14532 Kleinmachnow (DE) - +49-3320348377 - +49-3320348385 - petra.mueller@jki.bund.de
Nemeth Jozsef (Mr)
Plant Protection and Soil Conservation Service, P.O. Box 13, 7615 Pecs (HU) - +36-72552246 - +36-72255940 - nemeth.jozsef@baranya.ontsz.hu
Özakman Meriç (Mr)
Plant Protection Central Research Institute, Bagdat Cad. No:250, 06172 Yenimahalle-Ankara (TR)
+90-3123445994 ext 159 - +90-3123151531 - m_ozakman@yahoo.com
Sletten Arild (Mr)
Norwegian Institute for Agricultural and Environmental Research, Plant Health and Plant Protection Division, Hogskoleveien 7, N-1432 Aas (NO) - +47-90526644 - +47-64946110 - arild.sletten@bioforsk.no
Stead David (Mr)
Central Science Laboratory, 04FA09 Sand Hutton, Y04 1LZ York (GB) - +44-1904462336 - +44-190446225 - d.stead@csl.gov.uk
Stefani Emilio (Mr)
Dept. Agricultural Sciences, University of Modena & Reggio Emilia, Via J.F. Kennedy 17, 42100 Reggio Emilia (IT) - +39-0522522013 - +39-0522522053 - emilio.stefani@unimore.it
Tjou-Tam-Sin Napoleon (Mr)
National Reference Laboratory (NRL), Plant Protection Service, Wageningen (NL) - n.n.a.tjou-tam-sin@minlnv.nl
Ustun Nursen (Ms)
Plant Protection Research Institute, Genclik cad. No. 6, 35040 Bornova/Izmir (TR) - +90-2323880030 - +90-2323741653 - nursen_ustun@yahoo.com
Van Vaerenbergh Johan (Mr)
Institute for Agricultural and Fisheries Research (ILVO), Laboratory on bacterial diseases, CLO, Burg. Van Gansberghelaan 96, 9820 Merelbeke (BE) - +32-92722476 - +32-92722429 - johan.vanvaerenbergh@ilvo.vlaanderen.be
Petter Françoise (Ms)
McMullen Madeleine (Ms)
OEPP/EPPO, 1 rue Le Nôtre, 75016 Paris (FR) - +33 (0) 1 45 20 77 94 - +33 (0) 1 42 24 89 43 - hq@eppo.int
Observers
Koutzarova Eleonora (Ms)
Central laboratory for plant quarantine, 120, N. Moushanov Blvd., 1330 Sofia (BG) - +359-28 22 33 81 - +359 28 22 33 81 - e.kutzarova@nsrz.government.bg
Elphinstone John (Mr) - EAP
Central Science of Laboratory, Sand Hutton, YO41 1LZ York (UK) - j.elphinstone@csl.gov.uk
Janse Jaap (Mr) - EAP Chair
Dutch General Inspection Service (NAK) Department Laboratory Methods and Diagnosis, PO Box 1115, 6300 BC Emmeloord (NL) - +31-527635400 - +31-527635411 - jjanse@nak.nl
